Drinking-in-the-dark

'Drinking-in-the-Dark' (DID). Briefly, 3 hours into the dark cycle, home-cage water bottles are replaced with sippers containing 20% (v/v) unsweetened EtOH. Mice have free access to the EtOH-filled sippers for 2 hours. During this time, there is no water available. Food is always available ad libitum and mice have ad libitum access to water, except during this two-hour period each day. Mice are not water deprived. In this video, we also record 30 minutes of water consumption prior to the 2-hour EtOH period. Therefore, there is a 30 minute 'water baseline' followed by the 2-hour DID. In this video, these mice are on their second day of DID. Videos provided by Cherish Ardinger (Indiana University–Purdue University Indianapolis) and David Linsenbardt (University of New Mexico School of Medicine).